Biophysical Analytics

• Since 2018 W2 professorship for “Dynamic and Functional in vivo Imaging” at the Free University, Berlin, Veterinary Medicine
• 2013 Call for a W2 professorship for “Intravital Imaging in Immunology” at the Otto-von-Guericke University, Magdeburg / defended by the DRFZ, Berlin
• Since 2012 Leader of JIMI – a network for intravital imaging
• Since 2010 Group leader “Biophysical Analytics” and “Core-facility of advanced imaging” at the DRFZ, Berlin
• 2008 – 2010 Scientist (DFG grant of own position) at the Cecilie-Vogt Neurology Clinics, Charité, Berlin
• 2005 – 2008 PostDoc at the Helmholtz Center for Infection Research (HZI), Braunschweig
• 2001 – 2004 Doctoral student at the TU Braunschweig

• 2005 PhD in Biophysical Chemistry, mentor Prof. K.-H. Gericke, summa cum laude
• 2001 – 2004 PhD thesis at TU Braunschweig, Institute for Physical and Theoretical Chemistry
• 2001 Diploma thesis
• 1996 – 2001 Study of Chemistry at the University of Bucharest and at TU Braunschweig

• Member of committees for professorships calls at the Charité, Berlin
• Member of the steering committee of the integrated graduated school “B cells and beyond” of the CRC130
• Member of the steering committee of CTLS (Core Technologies for Life Sciences)
• Member of committees for professorships calls at the Institute for Physical and Theoretical Chemistry, TU Braunschweig

• International Society for Advancement in Cytometry
• American Biophysical Society
• Deutsche Gesellschaft für Zytometrie (Vice-President since 2018)
• Core Technologies for Life Sciences

• 2015 – 2019 Marylou-Ingram Scholar; International Society for Advancement in Cytometry
• 2009 – 2011 “Rachel-Hirsch” Habilitation fellowship of the Charité, Berlin
• 2008 – 2011 DFG grant for the own position
• 1998 Erasmus-Socrates fellowship / DAAD award for best foreign student

Selection from 2011 – ongoing

• 2012 – 2016 DFG NI1167/3-1 “JIMI – a network for intravital microscopy”(450.000 EUR)
• 2013 – 2021 DFG TRR130 “B cells: immunity and autoimmunity”, C01 “Intravital multi-photon microscopy and microendoscopy”, 1st and 2nd funding period (1.370.000 EUR)
• 2014 – 2020 DFG FOR2165 “Bone regeneration in aged individuals”, TP7, NI1167/4-1 +
  NI1167/4-2 (1st and 2nd funding period), “Longitudinal intravital imaging analysis of osteo-immune interactions during bone healing” (370.000 EUR)
• 2014 – 2016 BMWE ZIM “RETI-IM”, “Longitudinal imaging of the retina for dynamic and functional anal ysis of the central nervous system during neuroinflammation and neurodegeneration” (165.000 EUR)

1. Leben R, Köhler M, Radbruch H, Hauser AE, Niesner RA. Systematic Enzyme Mapping of Cellular Metabolism by Phasor-Analyzed Label-Free NAD(P)H Fluorescence Lifetime Imaging. IJMS, 2019, 20(22). pii: E5565.
2. Leben R, Ostendorf L, van Koppen S, Rakhymzhan A, Hauser AE, Radbruch H, Niesner RA. Phasor-Based Endogenous NAD(P)H Fluorescence Lifetime Imaging Unravels Specific Enzymatic Activity of Neutrophil Granulocytes Preceding NETosis. IJMS, 2018, 19(4). pii: E1018.
3. Reismann D, Stefanowski J, Günther R., Rakhymzhan A., Zehentmeier S, Nauendorf S, Bleek-Schmidt K., Chang H.-D., Winter Y, Duda G, Hauser A.E.*, Niesner R.A.*. Longitudinal Intravital Imaging of the Femoral Bone Marrow Reveals Plasticity Within Marrow Vasculature. Nature Comm., 2017, 8(1):2153.
4. Rakhymzhan A, Leben R, Zimmermann H, Günther R, Mex P, Reismann D, Ulbricht C, Acs A, Brandt AU, Lindquist RL, Winkler TH, Hauser AE, Niesner R. Synergistic Strategy for Multicolor Two-photon Microscopy: Application to the Analysis of Germinal Center Reactions In Vivo. Sci Rep. 2017 Aug; 7(1):7101.
5. Radbruch H, Bremer D, Guenther R, Cseresnyes Z, Lindquist R, Hauser AE, Niesner R. Ongoing Oxidative Stress Causes Subclinical Neuronal Dysfunction in the Recovery Phase of EAE. Front Immunol. 2016 Mar 14; 7:92.
6. Bremer D, Pache F, Günther R, Hornow J, Andresen V, Leben R, Mothes R, Zimmermann H, Brandt AU, Paul F, Hauser AE, Radbruch H, Niesner R. Longitudinal Intravital Imaging of the Retina Reveals Long-term Dynamics of Immune Infiltration and Its Effects on the Glial Network in Experimental Autoimmune Uveoretinitis, without Evident Signs of Neuronal Dysfunction in the Ganglion Cell Layer. Front Immunol. 2016 Dec 23; 7:642.
7. Mossakowski AA, Pohlan J, Bremer D, Lindquist R, Millward JM, Bock M, Pollok K, Mothes R, Viohl L, Radbruch M, Gerhard J, Bellmann-Strobl J, Behrens J, Infante-Duarte C, Mähler A, Boschmann M, Rinnenthal JL, Füchtemeier M, Herz J, Pache FC, Bardua M, Priller J, Hauser AE, Paul F, Niesner R*, Radbruch H*. Tracking CNS and systemic sources of oxidative stress during the course of chronic neuroinflammation. Acta Neuropathol. 2015 Dec;130(6):799-814.
8. Rinnenthal JL, Börnchen C, Radbruch H, Andresen V, Mossakowski A, Siffrin V, Seelemann T, Spiecker H, Moll I, Herz J, Hauser AE, Zipp F, Behne MJ, Niesner R. Parallelized TCSPC for dynamic intravital fluorescence lifetime imaging: quantifying neuronal dysfunction in neuroinflammation. PLoS One. 2013 Apr 16; 8(4):e60100.
9. Andresen V, Pollok K, Rinnenthal J-L, Oehme L, Günther R, Spiecker H, Radbruch H, Gerhard J, Sporbert A, Cseresnyes Z, Hauser AE*, Niesner R*. High resolution intravital microscopy. PLoS One. 2012; 7(12):e50915.
10. Siffrin V, Radbruch H, Glumm R, Niesner R, Paterka M, Herz J, Leuenberger T, Lehmann SM, Luenstedt S, Rinnenthal JL, Laube G, Luche H, Lehnardt S, Fehling HJ, Griesbeck O, Zipp F. In vivo imaging of partially reversible Th17 cell-induced neuronal dysfunction in the course of encephalomyelitis. Immunity. 2010 Sep 24; 33(3):424-36.
11. Herz J, Siffrin V, Hauser AE, Brandt AU, Leuenberger T, Radbruch H, Zipp F, Niesner RA. Expanding two-photon intravital microscopy to the infrared by means of optical parametric oscillator. Biophys J. 2010 Feb 17; 98(4):715-23.
12. Niesner R, Andresen V, Neumann J, Spiecker H, Gunzer M. The power of single and multi-beam two- photon microscopy for high-resolution and high-speed deep tissue and intravital imaging. Biophys J. 2007 Oct 1;93(7):2519-29

* equally contributing authors