Cell sorting without dyes

Researchers at the DRFZ have developed an innovative method for cell sorting. Using Multi-Angle Pulse Shape (MAPS) flow cytometry, cells can be sorted based on their scattered light properties without the need for fluorescent markers. This method has already made it possible to analyse cell cycle phases without the need for traditional cell cycle dyes. Toralf Kaiser (technical head of the flow cytometry core facility, FCCF) and his team has now further improved the technique so that cells can not only be analysed but also sorted for further functional studies. In addition, different immune cell types, such as T and B cells, can also now be distinguished without the use of dyes. These results were recently published in the journal Communications Biology.

The analysis and sorting of specific cell types or cells in different physiological states is essential for biomedical research. Until now, cells have often been identified using reagents that specifically stain them and make them visible for optical measurement techniques. However, this new dye-free method promises a wider range of applications, particularly in research of cellular functions and disease processes, as it simplifies sample preparation and the results are not affected by labeling dyes.